DNA-free induction of sequence-specific mutations in Vicia faba using the CRISPR/Cas system

Cultivation of legumes will become more important in sustainable as well as in conventional agriculture even in Germany and Europe. As a model plant for legumes, the project focuses on the domestic grain legume field bean (Vicia faba). Convincing arguments for the field bean are a high yield potential, its effect as 'break crop' in cereal-rich crop rotations, its favourable effect on soil fertility and its symbiosis performance. Especially breeding successes in yield stability would be able to advance the cultivation of this crop. Modern techniques such as genome editing must not be disregarded when it comes to breeding progress. So far, biotechnological approaches could not be used for the breeding of field beans due to their poor suitability for tissue culture. Vicia faba has a low morphogenic potential and its tissue tends to brown quickly. To date, only one tissue of faba bean with a high morphogenic potential has been identified: the embryonic axis of the mature embryo and explanates derived from this tissue. The aim of this project is to establish genome editing with the help of the CRISPR/Cas system in field beans. The components of the system will be introduced DNA-free as a ribonucleoprotein complex via particle bombardment into the regenerative tissue of the embryonic axis. The project is funded by the BMBF within the framework of the call for proposals "Cultivated Plants of the Future".


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