Geschäftsführung / Institutsleitung

Die Geschäftsführung der RLP AgroScience GmbH erfolgt durch zwei Geschäftsführer, Prof. Dr. G. Krczal und Prof. Dr. R. Kubiak. Jeder der Geschäftsführer leitet darüber hinaus eines der Institute unter dem Dach der RLP AgroScience GmbH

Institutsleitung AlPlanta - Institut für Pflanzenforschung

Prof. Dr. G. Krczal

Veröffentlichungen

Feldhoff, A., Kikkert, M., Kormelink, R., Krczal, G., Goldbach, R., and Peters, D., 1997: Serological comparison of tospoviruses with polyclonal antibodies produced against the main structural protein of tomato spotted wilt virus (TSWV), Archivs of Virology, 142, 781-793.

Feldhoff, A., Wetzel, T., Peters, D., Kellner, R. & Krczal, G. (1998): Characterization of a petunia flower mottle virus (PetFMV), a new potyvirus infecting Petunia x hybrida. Archivs of Virology 143, 475-488.

Krczal, G. and Wetzel, T. (1999) An immunocapture/PCR method for the detection of petunia flower mottle potyvirus in petunias. Petria 9, 141-143.

Tang, H., Zhenglong, R., Krczal, G (2000). Somatic Embryogenesis and Organogenesis from immature Embryo Cotyledons of three Sour Cherry Cultivars (Prunus cerasus L.), Scientia Horticulturae, 83, 2, 109-126

Tang, H., Zhenglong, R., Krczal, G (2000) Improvement of English Walnut Somatic Embryo Germination and Conversion by Dessication Treatments and Plantlet Development by Lower Medium Salts, In Vitro Cell Dev. Biol.-Plant, 3647-50

Tang, H., Ren, Z. and Krczal, G. (2000): An evaluation of antibiotics of Agrobacterium tumefaciens from walnut somatic embryos and for the effects on the proliferation of somatic embryos and regeneration of transgenic plants. Plant Cell Reports, 19, 881-887.

Tang, Haoru, Wang, Yongquing, Ren Zhenglong and G. Krczal (2000): Somatic Embryo- genesis and Plant Regeneration from Embryonic Axes and Cytyledons of Walnut Immature Embryos of CV. No. 120. Acta Horticulturae Sinica, Vol. 27, 59-61

Galetzka, D., Russo, M, Rubino, L. and Krczal, G. (2000): Molecular Characterization of a Tombusvirus associated with a disease of Statice (Goniolimon tataricum (L.) Boiss.). Journal of Plant Pathology, 82 (2), 151-155.

Wetzel, T., Moerbel, J. and Krczal, G (2000): A ligation-anchored PCR mezhod for the cloning of the 3´end of a tobamovirus infecting Impatiens New Guinea hybrids. EPPO Bulletin, 30, 449-451.

T. Wetzel, L. Meunier, U. Jäger, G.M. Reustle, G. Krczal (2001): Complete nucleotide sequences of the RNA's 2 of German isolates of grapevien fanleaf and Arabis mosaic nepoviruses. Virus Research 75, 139-145.

Wetzel, T. Jardak, R. Meunier, L., Ghorbel, A. Reustle, G.M. and Krczal, G. (2002): Simultaneous RT/PCR detection and differentiation of arabis mosaic and grapevine fanleaf nepoviruses in grapevines with a single pair of primer, Journal of Virological Methods, 101, 63-69.

Wetzel, T., Fuchs, M., Bobko, M. and Krczal, G. (2002) Size and sequence variability of the arabis mosaic virus protein 2A. Arch. Virol., 147, 1643-1645.

Ebel, R., Schnabel, A., Reustle, G.M., Krczal, G. and Wetzel, T. (2003) Complete nucleotide sequence of an isolate of the nepovirus raspberry ringspot virus from grapevine. Virus Res. 97 (2), 141-144.

Wetzel, T., Beck., A., Wegener, U., and Krczal, G. (2004) Complete nucleotide sequence of the RNA 1of a grapevine isolate of arabis mosaic virus. Arch. Virol., 149, 989-995.

Boonrod, K., Galetzka, D., Nagy, PD., Conrad, U and Krczal, G. (2004) Single-chain antibodies against a plant viral RNA-dependent RNA polymerase confer virus resistance. Nature Biotechnol., 22 (7), 856-862.

Boonrod, K., Chotewutmontri, S., Galetzka, D. and Krczal, G. (2005) Analysis of revertants to identify amino acid residues within RNA-dependent RNA polymerase motifs. J. Gen. Virol., 86, 823-826.

Boonrod, K., Moser, M. and Krczal, G. (2005) Chloroform phase partitioning for purification of plant-expressed proteins. Anal. Biochem., 344, 150-151

Wetzel, T., NJapo NGangom, H.O., Chotewutmontri, S., and Krczal, G. (2006) Nucleotide sequence of a new isolate of Ribgrass mosaic tobamovirus infecting Impatiens New Guinea. Arch. Virol., 151(4), 787-791.

Wetzel, T., Ebel, R., Moury, B., Le Gall, O., Endisch, S., Reustle, G.M., and Krczal, G. (2006) Sequence analysis of grapevine isolates of Raspberry ringspot nepovirus. Arch. Virol, 151(3), 599-606.

Wetzel, T., Bassler, A., Wahab, M.A.B.A., and Krczal G. (2006) A RT/PCR-partial restriction enzymatic mapping (PREM) method for the molecular characterisation of the large satellite of Arabis mosaic virus, J. Virol. Meth., 132(1-2),97-103.

Wassenegger M. and Krczal G. (2006). Nomenclature and functions of RNA-directed RNA polymerases. Trends Plant Sci., 11: 142-151.

Ben Amar, A., Cobanov, P., Boonrod, K., Krczal, G., Bouzid, S., Ghorbel, A. Reustle, G. M. (2007):Efficient Procedure for Grapevine Embryogenic Suspension Establishment and Plant Regeneration: Role of Conditioned Medium for Cell Proliferation, Plant Cell reports, 26 (9), 1439-1447.

Wetzel, T. and Krczal, G. (2007) Molecular Biology of Raspberry ringspot nepovirus. Plant Viruses 1(1), 45-51.

Ben-Amar, A., Bassler, A., Ghorbel, A., Mliki, A., Krczal, G., and Wetzel, T. (2007): Determination of the length of the poly(A) tails of the Arabis mosaic nepovirus genomkic RNAs. Plant Viruses, 1(2), 208-210

Heim, F., Lot, H., Delecolle, B., Bassler, A., Krczal, G., and Wetzel, T. (2008) Complete nucleotide sequence of a new rhabdovirus infecting lettuce, Archiv. Virol., 153, 81-92

Dupuis, L., Cobanov, P., Bassler, A., Krczal, G., and Wetzel, T. (2008) Completegenome sequences of a virulent isolate of Arabis mosaic virus from privet (Ligustrum vulgare). Arch. Virol.,  153, 1611-1613

Schwind N., Zwiebel M., Itaya A., Ding B., Krczal G. and Wassenegger M. (2008). RNAi-mediated resistance against potato spindle tuber viroid (PSTVd) in tomato and Nicotiana tabacum. Mol. Plant Pathol. 10, 459-469.

Isabel Weinheimer; Kajohn Boonrod; Mich?le Zwiebel; Marc Füllgrabe; Gabi Krczal; Michael Wassenegger (2009) Analysis of an auto-proteolytic activity of rice yellow mottle virus silencing suppressor P1, Biol Chem, submitted

Boonrod K. and Krczal G. (2009). Inhibition of Positive-Sense (ss) RNA Viruses RNA-Dependent RNA Polymerases. Curr Enzyme Inhibition 5: 234-244.

Boonrod K., Roth B., Leong Ngar S., Krczal G. (2009). Analysis of the effectiveness of reused primary and secondary antibodies in Western blotting analysis. Anal Biochem 397: 124-5. Epub 2009 Sep 12.

Dalakouras A., Moser M., Zwiebel M., Krczal G., Hell R. and Wassenegger M. (2009). A hairpin RNA construct residing in an intron efficiently triggered RNA-directed DNA methylation in tobacco. Plant J 60: 840-851.

Dalakouras A., Moser M., Krczal G. and Wassenegger M. (2009). A chimeric satellite transgene sequence is inefficiently targeted by viroid-induced DNA methylation. Plant Mol Biol, under reviewing.

Schwind N., Zwiebel M., Itaya A., Ding B., Wang M.B., Krczal G. and Wassenegger M. (2009). RNAi-mediated resistance to Potato spindle tuber viroid in transgenic tomato expressing a viroid hairpin RNA construct. Mol Plant Pathol 10: 459-469.

Wallbraun M., Sonntag K., Eisenhauer C., Krzcal G., and Wang Y.P. (2009). Phosphomannose-isomerase (pmi) gene as a selectable marker for Agrobacterium-mediated transformation of rapeseed. Plant Cell, Tissue and Organ Culture 99: 345-361.

Dalakouras A., Moser M., Krczal G. and Wassenegger M. (2010). A chimeric satellite transgene sequence is inefficiently targeted by viroid-induced DNA methylation in tobacco Plant Mol. Biol. 73: 439-447.

Weinheimer I., Boonrod K., Moser M., Zwiebel M., Füllgrabe M., Krczal G. and Wassenegger M. (2010). Analysis of an auto-proteolytic activity of riceyellow mottle virus silencing suppressor P1. Biol. Chem. 391,271-281.

Füllgrabe M., Boonrod K., Jamous R., Moser M., Shiboleth Y., Krczal G.. and Wassenegger M. (2010). Expression, purification and functional characterization of recombinant Zucchini yellow mosaic virus HC-Pro. Protein Expr. Purif., 75: 40-45

Dalakouras A., Moser M., Boonrod K., Krczal G., Wassenegger M. (2011). Diverse spontaneous silencing of a transgene among two Nicotiana species. Planta, DOI 10.1007/s00425-011-1433-9.  (Download)

Jamous R. M., Boonrod K., Fuellgrabe M. W., Ali-Shtayeh M. S., Krczal G. and Wassenegger M. (2011). The HC-Pro of the Zucchini Yellow Mosaic Virus (ZYMV) inhibits HEN1 methytransferase activity in vitro. J. Gen. Virol., in press.

Book contributions:

Krczal, G. (1998): Virus Certification of Ornamental Plants - The European Strategy. In: Plant Virus Disease Control, A. Hadidi, R.K. Khetarpal and H. Koganezawa, eds., APS Press, The American Phytopathological Societ, St. Paul, Minnesota

Krczal, G. (1999): Pflanzenzucht, in: Gentechnologie, Ullsteinverlag, Herausgeber D. Harreus.

Patents:

Patent application, Deutsches Patentamt

Boonrod KJ., Krczal G., Verfahren zur Expression von gegen virale Proteine gerichtete scFv-Fragmenten in Pflanzen und deren Verwendung

Patent application, European Patent

Wassenegger M., Krczal G., Dalakouras T. "Establishment of an intron-based in vivo DNA methylation systems to generate transgene-free plants displaying altered gene expression patterns"

Patent application, European Patent

Füllgrabe, M. Wassenegger, M., Boonrod, KJ, Krczal, G. Method for isolating small RNA-molecules using HC-Pro protein

 


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