ESTABLISHMENT OF EMBRYOGENIC CELL CULTURES FROM GRAPEVINES

 AND THEIR TRANSFORMATION FOR FUNCTIONAL GENE ANALYSIS

Genome sequencing and grapevine transcriptome analyses have generated a wealth of data on the structure and regulation of genes, which function, however, is often unclear. In addition to transient transformation systems (transient expression of the genes) for temporary overexpression or silencing of genes in grapevine using RNAi (RNA interference) technology, it is above all the production of stable transformants that is of great importance for the systematic analysis of gene functions in the intact plant. A prerequisite for the use of this strategy is the provision of efficient transformation and regeneration systems for grapevine. 

AlPlanta has developed a system for the establishment of highly productive embryogenic cell suspensions for different grapevine varieties and rootstocks. Embryogenic cultures of various cultivars of both quality varieties and rootstocks are maintained and can be effectively stimulated by the formation of somatic embryos to regenerate intact plants. These embryogenic grapevine cell cultures are the basis for the transformation for functional gene analysis, which is also offered as a service by us. Either the biolistic or the Agrobacteria-mediated approach can be chosen.

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