Publications

2018

- Dalakouras  A., Lauter A., Bassler A., Krczal G., Wassenegger M. (2018). Transient expression of intron-containing transgenes generates non-spliced aberrant pre-mRNAs that are processed into siRNAs. Planta 249:457-468.

- Dalakouras A., Wassenegger M., Dadami E., Ganopoulos I., Pappas M., Papadopoulou K.K. GMO-free RNAi: exogenous application of RNA molecules in plants. Plant Physiol 2019, doi: 10.1104/pp.19.00570.

- Jarausch, W., Runne, M., Wassenegger, M., Krczal, G. (2018). RNAi-mediated control of spotted wing drosophila (Drosophila suzukii): efficacy challenges and biosafety considerations. GMOs in integrated plant production, IOBC-WPRS Bulletin, 131: 51-55.

- Dalakouras A., Jarausch W., Buchholz G., Bassler A., Braun M., Manthey T., Krczal G. and Wassenegger M. (2018). Delivery of hairpin RNAs and small RNAs into woody and herbaceous plants by trunk injection and petiole absorption. Front Plant Sci 9: 1253.

2017

- Elena Dadami, Athanasios Dalakouras and Michael Wassenegger (2017), Viroids and RNA silencing. Viroids and Satellites: 115-124

- Abbas Q., Amin I., Mansoor S., Shafiq, M., Wassenegger M. and Briddon R.W. (2017). The Rep proteins encoded by alphasatellites restore expression of a transcriptionally silenced green fluorescent protein transgene in Nicotiana benthamiana. Virus Dis, doi.org/10.1007/s13337-017-0413-5

2016

- Dalakouras A., Wassenegger M., McMillan J., Cardoza V., Maegele I., Dadami D., Runne M., Krczal G. and Wassenegger M. (2016). Induction of silencing in plants by high-pressure spraying of in vitro-synthesized small RNAs. Front Plant Sci 7: 1327.

- Dalakouras A., Dadami E., Wassenegger M., Krczal G., Wassenegger M. (2016). RNA-directed DNA methylation efficiency depends on trigger and target sequence identity. Plant J. 87: 202-214.

2015

- Saeed M., Briddon R.W., Dalakouras A., Krczal G. and Wassenegger M. (2015). Functional analysis of Cotton Leaf Curl Kokhran Virus/Cotton Leaf Curl Multan Betasatellite RNA silencing suppressors. Biology 4: 697-714.

- Dalakouras A., Dadami E., Bassler A., Zwiebel M., Krczal G, Wassenegger M. (2015). Replicating Potato spindle tuber viroid mediates de novo methylation of an intronic viroid sequence but no cleavage of the corresponding pre-mRNA. RNA Biol. 12: 268-275.

- Dalakouras A., Dadami E. and Wassenegger M. (2015). Engineering Viroid Resistance. Viruses 7: 634-646.

- Saeed M., Krczal G. and Wassenegger M. (2015). Three gene products of a begomovirus–betasatellite complex restore expression of a transcriptionally silenced green fluorescent protein transgene in Nicotiana benthamiana.Virus Genes 50: 340-344.

2014

- Dadami E., Dalakouras A., Zwiebel M., Krczal G. and Wassenegger M. (2014). An endogene-resembling transgene is resistant to DNA methylation and systemic silencing. RNA Biol. 11: 934-941.

- Eamens A.L., Smith N. A., Dennis E.S., Wassenegger M., Wang M.-B. (2014). In Nicotiana species, an artificial microRNA corresponding to the virulence modulating region of Potato spindle tuber viroid directs RNA silencing of a soluble inorganic pyrophosphatase gene and the development of abnormal phenotypes. Virology 450-451: 266-277.

- Weinheimer I., Boonrod K., Moser M., Wassenegger M., Krczal G., Butcher S. and Valkonen J.P.T. (2014). Binding and processing of small dsRNA molecules by the class 1 RNase III protein encoded by Sweet potato chlorotic stunt virus. J. Gen. Virol. 95: 486-495.

2013

- Dalakouras A., Dadami E. and Wassenegger M. (2013). Viroid-induced DNA methylation in plants. Biomol. Concepts 4: 557-565.

- Dalakouras A. and Wassenegger M. (2013). Revisiting RNA-directed DNA methylation. RNA Biol. 10: 453-455.

- Dadami E., Moser M., Zwiebel M., Krczal G., Wassenegger M., Dalakouras A. (2013). An endogene-resembling transgene delays the onset of silencing and limits siRNA accumulation. FEBS Letters 587: 706–710.

2012

- Dalakouras A., Dadami E., Zwiebel M., Krczal G. and Wassenegger M. (2012). Transgenerational maintenance of transgene body CG but not CHG and CHH methylation. Epigenetics 7: 1071-1078.

2011

- Boonrod K., Füllgrabe M.W., Krczal G. and Wassenegger M. (2011). Analysis of the autoproteolytic activity of the recombinant helper component proteinase from zucchini yellow mosaic virus. Biol. Chem. 392: 937-945.

- Dalakouras A., Moser M., Boonrod K., Krczal G., Wassenegger M. (2011). Diverse spontaneous silencing of a transgene among two Nicotiana species. Planta 234: 699-707. (Download)

- Jamous R. M., Boonrod K., Fuellgrabe M. W., Ali-Shtayeh M. S., Krczal G. and Wassenegger M. (2011). The HC-Pro of the Zucchini Yellow Mosaic Virus (ZYMV) inhibits HEN1 methytransferase activity in vitro. J. Gen. Virol. 92: 2222-2226.

- Füllgrabe M., Boonrod K., Jamous R., Moser M., Shiboleth Y., Krczal G. and Wassenegger M. (2011). Expression, purification and functional characterization of recombinant Zucchini yellow mosaic virus HC-Pro. Protein Expr. Purif. 75: 40-45. (Download)

- Dalakouras A., Tzanopoulou M., Tsagris M., Wassenegger M., and Kalantidis K. (2011). Hairpin transcription does not necessarily lead to efficient triggering of the RNAi pathway. Transgenic Res. 20: 293-304.

2010

- Dalakouras A., Moser M., Krczal G., Wassenegger M. (2010). A chimeric satellite transgene sequence is inefficiently targeted by viroid-induced DNA methylation in tobacco. Plant Mol. Biol. 73: 439-447.

- Weinheimer I., Boonrod K., Moser M., Zwiebel M., Füllgrabe M., Krczal G., Wassenegger M. (2010). Analysis of an autoproteolytic activity of rice yellow mottle virus silencing suppressor P1. Biol. Chem. 391: 271-281. (Download)

Vor 2010

- Dalakouras A., Moser M., Zwiebel M., Krczal G., Hell R. and Wassenegger M. (2009). A hairpin RNA construct residing in an intron efficiently triggered RNA-directed DNA methylation in tobacco. Plant J. 60: 840-851. (Download)

- Schwind N., Zwiebel M., Itaya A., Ding B., Wang M.-B., Krczal G. and Wassenegger M. (2009). RNAi-mediated resistance to Potato spindle tuber viroid in transgenic tomato expressing a viroid hairpin RNA construct. Mol. Plant Pathol. 10: 459-469.

- Prins M., Laimer M., Noris E., Schultz J., Wassenegger M. and Tepfer M. (2008). Strategies for antiviral resistance in transgenic plants. Mol. Plant Pathol. 9: 73-83.

- Verhoeven J. Th. J., Jansen C. C. C., Roenhorst J. W., Steyer S., Schwind N. and Wassenegger M. (2008). First Report of Solanum jasminoides Infected by Citrus exocortis viroid in Germany and the Netherlands and Tomato apical stunt viroid in Belgium and Germany. Plant Disease 92: 973.

- Shiboleth Y. M., Haronsky E., Leibman D., Arazi T., Wassenegger M., Whitham S. A., Gaba V. and Gal-On A. (2007) The Conserved FRNK Box in HC-Pro, a Plant Viral Suppressor of Gene Silencing, Is Required for Small RNA Binding and Mediates Symptom Development. J. Virol. 81: 13135-13148. (Download)

- Wassenegger M. and Krczal G. (2006). Nomenclature and functions of RNA-directed RNA polymerases. Trends Plant Sci. 11: 142-151.

- Wassenegger M. (2005). The role of the RNAi machinery in heterochromatin formation. Cell 122: 13-16. (Download)

- Qi Y., Pélissier T., Hunt E., Wassenegger M. and Ding B. (2004). A direct role of RNA structures in phloem-mediated systemic traffic. Plant Cell 16: 1741-1752. (Download)

- Vogt U., Pélissier T., Pütz A., Razvi F., Fischer R. and Wassenegger M. (2004). Viroid-induced RNA silencing of GFP-viroid fusion transgenes does not induce extensive spreading of methylation or transitive silencing. Plant J. 38: 107-118. (Download)

- Wassenegger M. (2002). Gene silencing-based disease resistance. Transgenic Res. 11: 639-653. (Download)

- Wassenegger M. (2002). Gene silencing. Internat. Rev. Cytol. 219: 61-113.

- Bonin M., Oberstrass J., Vogt U., Wassenegger M. and Nellen W. (2001). Binding of IRE-BP to its cognate RNA sequence: SFM studies on a universal RNA backbone for the analysis of RNA-protein interaction. Biol. Chem. 382: 1157-1162. (Download)

- Wassenegger M. (2001). Advantages and disadvantages of using PCR techniques to characterize transgenic plants. Mol. Biotech. 17: 73-82. (Download)

- Wassenegger M. (2000). RNA-directed DNA methylation. Plant Mol. Biol. 43: 203-220. (Download)

- Pélissier T. and Wassenegger M. (2000). A DNA target of 30 bp is sufficient for RNA-directed DNA methylation. RNA 6: 55-65. (Download)

- Bailey-Serres J., Rochaix J.-D., Wassenegger M. and Filipowicz W. (1999). Plants, their organelles, viruses and transgenes reveal mechanisms and relevance of post-transcriptional processes. EMBO J. 18: 5153-5158. (Download)

- Pélissier T., Thalmeir S., Kempe D., Sänger H. L. and Wassenegger M. (1999). Heavy de novo methylation at symmetrical and non-symmetrical sites is a hallmark of RNA-directed DNA methylation. Nucl. Acids Res. 27: 1625-1634. (Download)

- Wassenegger M. and Pélissier T. (1999). Signalling in gene silencing. Trends Plant Sci. 4: 207-209.

- Schiebel W., Pélissier T., Riedel L., Thalmeir S., Schiebel R., Kempe D., Lottspeich F., Sänger H. L. and Wassenegger M. (1998). Isolation of a RNA-directed RNA polymerase-specific cDNA clone from tomato leaf-tissue mRNA. Plant Cell 10: 2087-2101. (Download)

- Wassenegger M. and Pélissier T. (1998). A model for RNA-mediated gene silencing in higher plants. Plant Mol. Biol. 37: 349-362. (Download)

- Wassenegger M., Spieker R., Riedel L., Thalmeir S., Gast F.-U. and Sänger H. L. (1996). A single nucleotide substitution converts potato spindle tuber viroid (PSTVd) from a non-infectious to an infectious RNA for Nicotiana tabacum. Virology 226: 191-197. Download

- Riedel L., Volger U., Luckinger R., Pütz A., Sänger H. L. and Wassenegger M. (1996). Molecular analysis of the gene family of the signal recognition particle (SRP) RNA of tomato. Plant Mol. Biol. 31: 113-125.

- Riedel L., Pütz A., Hauser M.-T., Luckinger R., Wassenegger M. and Sänger H. L. (1995). Characterization of the SRP RNA population of tomato. Plant Mol. Biol. 27: 669-680.

- Wassenegger M., Heimes S. and Sänger H. L. (1994). An infectious viroid RNA replicon evolved from an in vitro-generated non-infectious viroid deletion mutant via a complementary deletion in vivo. EMBO J. 13: 6172-6177. (Download)

- Wassenegger M., Heimes S., Riedel L. and Sänger H. L. (1994). RNA-directed de novo methylation of genomic sequences in plants. Cell 76: 567-576.

 

Buchkapitel:
 -Gabi Krczal and Michael Wassenegger, Potyvirus Helper Component Proteinase. In Neil D. Rawlings and Guy S. Salvesen, editors: Handbook of Proteolytic Enzymes, Oxford: Academic Press , 2013 , pp. 2187 - 2192

- Müller A. and Wassenegger M. (2004). Control and silencing of transgene expression. In Handbook of Plant Biotechnology 2, Christou, P. and Klee, H. eds. (Wiley), pp 291-330.

- Wassenegger M. (1998). Application of PCR to transgenic plants. PCR in Bioanalysis. In Methods in Mol. Biol., 92, S.J. Meltzer (Humana Press Inc., Totowa), pp. 153-164.

- Sänger H. L., Schiebel W., Riedel L., Pélissier T. and Wassenegger M. (1996). The possible links between RNA-directed DNA methylation (RdDM), sense and antisense RNA, gene silencing, symptom-induction upon microbial infections and RNA-directed RNA polymerase (RdRP). In Biology of Plant-Microbe Interactions, G. Stacey, B. Mullin, and P.M. Gresshoff, eds. (St. Paul, MN: American Phytopathological Society), pp. 533-540.

 

Patente/Patentanmeldungen:
- US-Patent (Nr.: 6,218,142B1 und 7,091,397)

"Nucleic acid molecules encoding polypeptides having the enzymatic activity of an RNA-directed RNA polymerase (RdRP)". (Datum der Patenterteilung: 17. Apr. 2001)

- Europäische (Nr.: EP 2 198 700 A1) und ?Patent Cooperation Treaty? (PCT) (Nr.: WO 2010/066343 A1)

"Method for the production of a transgene-free plant with altered methylation pattern". (Datum der Anmeldung 09. Dez. 2008, Erteilt am 05. September 2012)

- Europäische (Nr.: 10005298.4)